Propranolol and verapamil inhibit mRNA expression of RyR2 and SERCA in L-thyroxin-induced rat ventricular hypertrophy

作     者：rat ventricular hypertrophy1 Xiao-dong WU2,De-zai DAI2,Qiu-pin ZHANG3,Feng GAO3 Research Division of Pharmacology,China Pharmaceutical University 3Laboratorial Center ofMedicine,Southeast University,Nanjing 210009,China 

作者机构：Research Division of Pharmacology China Pharmaceutical University Laboratorial Center of Medicine Southeast University Nanjing 210009 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第25卷第3期

页      面：93-97页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by the National Natural Science Foundationof China (No 39670835) 

主　　题：heart hypertrophy arrhythmia ryanodine receptors Ca2+-transporting ATPase L-thyroxin propranolol verapamil reversed transcriptase polymerase chain reaction 

摘      要：AIM: To study the alteration in the mRNA level of cardiac ryanodine receptor 2 (RyR2) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) in L-thyroxin-induced hypertrophy. METHODS: L-thyroxin (500 g/kg) daily was injected for 10 d. RT-PCR was used to determine mRNA expression. RESULTS: An increase in the relative amount of RyR2 (111 %) and SERCA mRNA (65 %) expression was observed in the hypertrophied rats (RyR2: 77±11; SERCA: 87±10, n=9) compared with the normal rats (RyR2: 36±10; SERCA: 53±10, n=9). Propranolol was effective to inhibit the increase in RyR2 (51±7) and SERCA (63±13) mRNA expression in hypertrophied rats, respectively. Verapamil also reduced RyR2 (62±5) and SERCA (75±8) mRNA expression. CONCLUSION: Both RyR2 and SERCA mRNA level in L-thyroxin-induced cardiac hypertrophy was over-expressed and propranolol or verapamil inhibited the alteration.