Bacterial diversity and distribution in the southeast edge of the Tengger Desert and their correlation with soil enzyme activities

作     者：Wei Zhang Gaosen Zhang Guangxiu Liu Zhibao Dong Tuo Chen Manxiao Zhang Paul J.Dyson Lizhe An 

作者机构：Key Laboratory of Desert and DesertificationCold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of SciencesLanzhou 730000China School of Life SciencesLanzhou UniversityLanzhou 730000China Institute of Life ScienceCollege of MedicineSwansea UniversitySwansea SA28PPUK 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2012年第24卷第11期

页      面：2004-2011页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No.31100365, 31170465,40971034) the International S&T Cooperation Program of China (No.2011DFA32520) the Science and Technology Projects in Gansu Province (No.1011FKCA114,1010RJZA009) 

主　　题：Tengger Desert DGGE 16S rRNA bacterial community soil enzyme 

摘      要：The nature of microbial communities and their relation to enzyme activities in desert soils is a neglected area of investigation. To address this, the bacterial diversity and distribution and soil physico-chemical factors were investigated in the soil crust, the soil beneath the crust and rhizosphere soil at the southeast edge of the Tengger Desert, using the denaturing gradient gel electrophoresis of 16S rRNA genes amplified by the polymerase chain reaction. Phylogenedc analysis of the sequenced DGGE bands revealed a great diversity of bacteria. The Proteobacteria, consisting of the α, β, and γsubdivisions, were clearly the dominant group at all depths and in rhizosphere soil. Analysis of the enzyme activities indicated that the rhizosphere soil of Caragana korshinskii exhibited the highest protease and polyphenol oxidase activities, and in the soil crust there were increased activities of catalase, urease, dehydrogenase and sucrase. The bacterial community abundance closely correlated with soil enzyme activities in different soils. The presence of Cyanobacteria correlated with significant increases in protease, catalase and sucrase in the soil crust, and increased urease in the rhizosphere soil of Artemisia ordosica. The occurrence of Acidobacteria was associated with significant increases in urease, dehydrogenase, and sucrase in the rhizosphere soil of C. korshinski. The presence of γ-Proteobacteria correlated with a significant increase in polyphenol oxidase in the rhizosphere soil ofA. ordosica. The study indicated a close relationship between the soil bacterial community and soil enzymes, suggesting the necessity of further investigations into bacterial function in this desert ecosystem.