Searching for the best agarose candidate from genus Gracilaria, Eucheuma,Gelidium and local brands

作     者：Ferry Efendi Retno Handajani Nursalam Nursalam 

作者机构：Faculty of NursingAirlangga University Department of Biochemistry MedicineFaculty of MedicineAirlangga University Institute of Tropical DiseaseAirlangga University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2015年第5卷第10期

页      面：865-869页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0907[农学-林学] 090705[农学-野生动植物保护与利用] 09[农学] 

基　　金：Supported by the Directorate General of Higher Education(Dirjen Dikti) Ministry of National Education of Indonesia through the “Penelitian Hibah Bersaing” scheme(Grant No.436/J)3.2/PG/2008) 

主　　题：Gracilaria agarose gigas imported migration candid 

摘      要：Objective: To explore the potential of local agar of genus Gracilaria, Eucheuma,Gelidium and local brands as an alternative for imported agarose for DNA electrophoresis, and to examine their ability related to separation and migration of DNA fragments in DNA ***: Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA ***: The local agar genus Gracilaria gigas, Gelidium, brand “B and brand “Scould separate DNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial ***: Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by p H and sulfur control.