Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis

作     者：Xiao-Huang Tu Jing-Xiang Song Xiao-Jun Xue Xian-Wei Guo Yun-Xia Ma Zhi-Yao Chen Zhong-Dong Zou Lie Wang 

作者机构：Department of General SurgeryFuzhou General Hospital of Nanjing Military RegionFuzhou 350025Fujian ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2012年第18卷第18期

页      面：2270-2279页

核心收录：

学科分类：090603[农学-临床兽医学] 08[工学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by Health and Medicine Scientific Research Foundation of Nanjing Military Area Command No.08Z029 

主　　题：Bone marrow mesenchymal stem cells Severe acute pancreatitis Intestinal barricade function Pancreatic acinar cells 

摘      要：AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time *** a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs *** AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into *** both studies,the protective effect of MSCs was ***:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time *** vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP *** SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 *** mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting ***:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic in