Electroacupuncture regulates the stress-injury-repair chain of events after cerebral ischemia/reperfusion injury

作     者：Peng Shi Lin-lin Sun Yi-shuo Lee Ya Tu 

作者机构：School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2017年第12卷第6期

页      面：925-930页

核心收录：

学科分类：0710[理学-生物学] 1005[医学-中医学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by a grant from the Major Science and Technology Project "Major New Drug Created" Funding No.2009ZX09103-707 

主　　题：repair acupuncture hematoxylin eosin minutes endogenous Acupuncture neurological inflammation staining 

摘      要：Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are released by damaged cells, which can lead to systemic stress reactions. Protecting the brain through repair begins with the stress-injury-repair signaling chain. This study aimed to verify whether acupuncture acts through this chain to facilitate effective treatment of ischemic stroke. Rat models of cerebral ischemia/reperfusion injury were established by Zea Longa＇s method, and injury sites were identified by assessing neurological function, 2,3,5-triphenyltetrazolium chloride staining, and hematoxylin-eosin staining. Electroacupuncture at acupoints Baihui（DU20） and Zusanli（ST36） was performed in the model rats with dilatational waves, delivered for 20 minutes a day at 2–100 Hz and an amplitude of 2 m A. We analyzed the blood serum from the rats and found that inflammatory cytokines affected the levels of adrenotrophin and heat shock protein 70, each of which followed a similar bimodal curve. Specifically, electroacupuncture lowered the peak levels of adrenocorticotrophic hormone and heat shock protein 70. Thus, electroacupuncture was able to inhibit excessive stress, reduce inflammation, and promote the repair of neurons, which facilitated healing of ischemic stroke.