Foliar Micromorphology of In Vitro-cultured Shoots and Field-grown Plants of Passiflora foetida

作     者：Manokari Mani Mahipal Singh Shekhawat 

作者机构：Department of BotanyKanchi Mamunivar Centre for Post Graduate Studies Biotechnology LaboratoryDepartment of Plant ScienceMahatma Gandhi Government College 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2017年第3卷第1期

页      面：34-40页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：financial support to our laboratory as Major Research Project and Grant-In-Aid Scheme (No. 10/DSTE/GIA/RP/JSA-I/2013/213) 

主　　题：Passiflora foetida micromorphology micropropagation stomata trichomes 

摘      要：The present report describes the development of quantitative and qualitative foliar micromorphological and architectural features in the field environment which elucidated the adaptation of micropropagated plants of Passiflora foetida L. in the natural soil conditions. The field environment(high light intensity in comparison to in vitro culture conditions) promotes the autotrophy through decrease in stomatal index(from 23.2 ± 0.15 to 21.0 ± 0.19), increased vein-islets(from 10.0 ± 0.14 to 15.6 ± 0.24 per square millimeters) and veinlet terminations(from 1.6 ± 0.14 to 5.0 ± 0.20 per square millimeters), and trichome density in P. foetida plantlets. The in vitro and field grown leaves mostly possessed anomocytic and anisocytic types of stomata. Two types of trichomes were observed on the surface of leaves of in vitro as well as field transferred plants of P. foetida: the unicellular hairy trichomes(non-glandular), and the multicellular(glandular) trichomes. The trichomes density was less under in vitro conditions as compared to the in vivo environments. The new leaves formed during the ex vitro rooting stage(in greenhouse) and after transplantation of plantlets to the field exhibited the development of adaptive micromorphological features in micropropagated plants, which enabled them to survive under field conditions.