A Complete Embryonic Developmental Table of Microhyla fissipes(Amphibia,Anura,Microhylidae)

作     者：Shouhong WANG Lanying ZHAO Lusha LIU Dengwei YANG Janak Raj KHATIWADA Bin WANG Jianping JIANG 

作者机构：Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Asian Herpetological Research》 (亚洲两栖爬行动物研究（英文版）)

年 卷 期：2017年第18卷第2期

页      面：108-117页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 0905[农学-畜牧学] 09[农学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 071002[理学-动物学] 

基　　金：supported by Important Research Project of Chinese Academy of Sciences(KJZG-EW-L13) National Natural Science Foundation of China(NSFC No.31471964) 2015 Western Light Talent Culture Project of the Chinese Academy of Sciences(Y6C3021) the Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization of CIB,CAS the Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province 

主　　题：Microhylidae embryonic stage external characters morphogenesis 

摘      要：Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches（range 209–564 eggs） of ornamented pygmy frog Microhyla fissipes（Amphibia, Anura, Microhylidae） were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated： 1） early embryonic development period（stages 1–28）, from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature（WT） 23–25℃; 2） larval development period（stages 29–45）, from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5℃, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease.