Comparison of the In Vitro and In Vivo Toxic Effects of Three Sizes of Zinc Oxide(ZnO)Particles Using Flounder Gill(FG)Cells and Zebrafish Embryos

作     者：HAN Li ZHAI Yanan LIU Yang HAO Linhua GUO Huarong 

作者机构：Key Laboratory of Marine Genetics and Breeding(Ocean University of China)Ministry of EducationCollege of Marine Life SciencesQingdao 266003P.R.China Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdao 266003P.R.China First Institute of OceanographyState Oceanic AdministrationQingdao 266061P.R.China 

出 版 物：《中国海洋大学学报：英文版》 (Journal of Ocean University of China)

年 卷 期：2017年第16卷第1期

页      面：93-106页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 09[农学] 0815[工学-水利工程] 0903[农学-农业资源与环境] 0824[工学-船舶与海洋工程] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.41076075,31472274 and 31172391) Scholarship Foundation for Excellent Scientists of Shandong Province(Grant No.BS2011 SW054) open foundation from Institute of Marine Biodiversity and Evolution(Grant No.20132017) 

主　　题：zinc oxide (ZnO) nanoparticles cytotoxicity embryotoxicity zebrafish fish cells 

摘      要：Nano-sized zinc oxide(n ZnO) particles are one kind of the most commonly used metal oxide nanoparticles(NPs). This study compared the cytotoxic and embryotoxic effects of three increasing sized ZnO particles(φ30 nm, 80-150 nm and 2 μm) in the flounder gill(FG) cells and zebrafish embryos, and analyzed the contribution of size, agglomeration and released Zn^(2+) to the toxic effects. All the tested ZnO particles were found to be highly toxic to both FG cells and zebrafish embryos. They induced growth inhibition, LDH release, morphological changes and apoptosis in FG cells in a concentration-, size-and time-dependent manner. Moreover, the release of LDH from the exposed FG cells into the medium occurred before the observable morphological changes happened. The ultrasonication treatment and addition of serum favored the dispersion of ZnO particles and alleviated the agglomeration, thus significantly increased the corresponding cytotoxicity. The released Zn^(2+) ions from ZnO particles into the extracellular medium only partially contributed to the cytotoxicity. All the three sizes of ZnO particles tested induced developmental malformations, decrease of hatching rates and lethality in zebrafish embryos, but size-and concentration-dependent toxic effects were not so obvious as in FG cells possibly due to the easy aggregation of ZnO particles in freshwater. In conclusion, both FG cells and zebrafish embryos are sensitive bioassay systems for safety assessment of ZnO particles and the environmental release of ZnO particles should be closely monitored as far as the safety of aquatic organisms is concerned.