Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma

作     者：Dhiraj M. Rathod Keyur R. Patel Hiren N. Mistri Arvind G. Jangid Pranav S. Shrivastav Mallika Sanyal 

作者机构：Department of Chemistry Kadi Sarva Vishwavidyalaya Gandhinagar Ahmedabad 382015 India Bioanalytical Research Department Accutest Research Lab Bodakdev Ahmedabad 380059 India Department of Chemistry School of Sciences Gujarat University Ahmedabad 380009 India Department of Chemistry St. Xavier's College Navrangpura Ahmedabad 380009 India 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2017年第7卷第1期

页      面：56-62页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 10[医学] 0702[理学-物理学] 

基　　金：the support and necessary facilities provided by Accutest Research Lab  Ahmedabad  for this work 

主　　题：Allopurinol Oxypurinol Liquid chromatography–tandem mass spectrometry Bioequivalence study 

摘      要：The present study describes a simple, reliable and reproducible liquid chromatography–tandem mass spectrometry method(LC–MS/MS) for the simultaneous determination of allopurinol and its active metabolite,oxypurinol in human plasma for a pharmacokinetic/bioequivalence study. After protein precipitation(PPT) of100 μL plasma sample with 1.0% formic acid in acetonitrile, the recovery of the analytes and allopurinol-d2 as an internal standard ranged from 85.36% to 91.20%. The analytes were separated on Hypersil Gold(150 mm×4.6 mm, 5 μm) column using 0.1% formic acid-acetonitrile(98:2, v/v) as the mobile phase.Quantification was done using electrospray ionization in the positive mode. The calibration concentration range was established from 60.0 to 6000 ng/m L for allopurinol and 80.0–8000 ng/m L for oxypurinol. Matrix effect in human plasma, expressed as IS-normalized matrix factors ranged from 1.003 to 1.030 for both the analytes. The developed method was found suitable for a clinical study with 300 mg allopurinol tablet formulation in healthy subjects.