Genetic biosensors for small-molecule products： Design and applications in high-throughput screening

作     者：Qingzhuo Wang Shuang-Yan Tang Sheng Yang 

作者机构：Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Shanghai 200025 China University of the Chinese Academy of Sciences Beijing 100049 China CAS Key Laboratory of Microbial Physiological and Metebolic Engineering Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China Jiangsu National Synergetic Innovation Center for Advanced Materials Nanjing Tech University Nanjing 211816 China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2017年第11卷第1期

页      面：15-26页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0817[工学-化学工程与技术] 08[工学] 080202[工学-机械电子工程] 09[农学] 071007[理学-遗传学] 0703[理学-化学] 0901[农学-作物学] 0802[工学-机械工程] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：biosensor small molecule product transcrip-tion factor riboswitch high-throughput screening 

摘      要：Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the in vivo concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into in vivo biosensors of small molecules, which couple the intracel- lular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screen- ing of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.