Mesoporous silica nanoparticles-assisted ruthenium(Ⅱ) complexes for live cell staining

作     者：Jia Wen Hui Yan Pengyi Xia Yongqian Xu Hongjuan Li Shiguo Sun 

作者机构：Shaanxi Key Laboratory of Natural Products & Chemical Biology School of Chemistry & Pharmacy Northwest Agriculture and Forestry University Yangling 712100 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2017年第60卷第6期

页      面：799-805页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the Scientific Research Foundation of Northwest A&F University (Z111021103, Z111021107) the National Natural Science Foundation of China (21472016, 21272030, 21476185) 

主　　题：ruthenium complexes mesoporous silica DNA imaging 

摘      要：Ruthenium complexes which can bind to DNA via electrostatic and intercalation interactions producing strong luminescence have become ideal candidates for DNA staining. However, some of them such as Ru(phen)_3Cl_2 and Ru(phen)_2(dppz)Cl_2 could hardly cross the cellular membrane of live cells which limited their further interaction with DNA in live cells. To solve this problem, a potential approach is to find a proper vehicle for loading and delivery of these ruthenium complexes into live *** silica nanoparticles(MSNs) with non-toxicity and good biocompatibility can be good candidates. More importantly,ruthenium complexes with positively charge could be loaded on negatively charged MSNs via electrostatic attractions to form MSNs-Ru hybrid. In vitro test demonstrated that MSNs had no side effects on the interactions between Ru complexes and ***, it is found that the MSNs-Ru hybrid can enter into living human cervical cancer cells HeLa and stain the DNA while the corresponding ruthenium complexes alone could hardly cross the cellular membrane in the control experiment, demonstrating MSNs can be employed to be an efficient ruthenium complexes delivery nanomaterial for live cell staining.