Hexabromocyclododecane-induced Genotoxicity in Cultured Human Breast Cells through DNA Damage

作     者：LI Rui Jing GAO Hui NA Guang Shui LU Zi Hao YAO Yao YANG Fan 

作者机构：Key Laboratory for Ecological Environment in Coastal AreasNational Marine Environmental Monitoring Center Dalian 116023LiaoningChina Shanghai Ocean UniversityShanghai 201306China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2017年第30卷第4期

页      面：296-300页

核心收录：

学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.41406088) The open fund of Key Laboratory for Ecological Environment in Coastal Areas,State Oceanic Administration(201506) 

主　　题：DNA HBCD Hexabromocyclododecane-induced Genotoxicity in Cultured Human Breast Cells through DNA Damage 

摘      要：To investigate the genotoxicity and reveal the potential toxicological mechanisms of Hexabromocyclododecane （HBCD）, human breast cells HBL-100 were exposed to a sequence of HBCD concentrations （0, 5, 10, and 50 mg/L） for 24 h. With a series of zymology and molecular biology methods, we found that HBCD induced dose-dependent oxidative stress on HBL-100 DNA. As revealed in q RT-PCR, activated prognostic factor ATM down-regulated tumor suppressor gene BRCA1 and prompted DNA repair genes h OGG1 and h MTH1 expression in lower concentrations of HBCD （〈 10 mg/L）. However, DNA repair were inhibited as well as cell proliferation rate by higher concentrations of HBCD （50 mg/L）. The results inferred that the genotoxicity of HBCD was dose-dependent and related to DNA repair pathway.