Characterisation of Class 1 Integron among <i>Escherichia coli</i>Isolated from Mansoura University Hospitals in Egypt
Characterisation of Class 1 Integron among <i>Escherichia coli</i>Isolated from Mansoura University Hospitals in Egypt作者机构：Microbiology Department Faculty of Pharmacy Mansoura University Mansoura Egypt
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2015年第5卷第4期
页 面：269-277页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Integron Antimicrobial Resistance E. coli RFLP
摘 要：Extensive use of antibacterials in clinical practice has been associated with increasing frequency of multi-resistant E. coli strains. Genetic elements such as Class 1 integrons have an important role in resistance development. In the current study, a total of 84 E. coli clinical isolates from Mansoura hospitals patients in Egypt were screened for antibacterial susceptibility against 12 different antibacterials. High resistance rates were identified for Ampicillin (92.9%) and sulfamethoxazole/ trimethoprim (84.5%). Class 1 integron was investigated in E. coli isolates by PCR. As a result, Class 1 integron was identified in 51.2% of these isolates. The contents of amplified integron varriable regions, were digested by Alu I restriction endonuclease. Cluster analysis of Class 1 integron digested varriable regions revealed that RFLP digested fragments generated could be classified into 9 different patterns, namely A, B, C, D, E, F, G, H and I. The most prevalent genotype was identifed in Group D. PCR and sequencing were used for detection of antimicrobial resistance genes harbored in integrons of Group D. As a result, main phylogenetic group identified harbored integron cassette carrying resistance gene for two antimicrobial groups namely aminoglycoside and trimethoprim. Multiresistance profiles in Group D exhibited association between antimicrobial resistance and integron presence. These findings suggest that the strategy for treatment of patients with E. coli infections needs to be revised. Furthermore, the high prevalence of Class 1 integron carrying gene confirms the importance of integron-mediated antimicrobial gene cassettes.
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