Elevated Birth Rates in CD62L (L-Selectin)-Deficient BALB/c Mice: Potential Involvement of NK Cells

作     者：Balint Farkas Jozsef Bodis Aaron R. Mangold Adrienn Angyal Ferenc Boldizsar Katalin Mikecz Tibor T. Glant 

作者机构：Department of Obstetrics and Gynecology University of Pecs Clinical Center Pécs Hungary Section of Molecular Medicine Department of Orthopedic Surgery Biochemistry Internal Medicine (Section of Rheumatology) at Rush University Medical Center Chicago USA Janos Szentagothai Research Centre University of Pecs Pécs Hungary Department of Dermatology Mayo Clinic Scottsdale USA Department of Cardiovascular Science University of Sheffield Sheffield UK Department of Immunology and Biotechnology University of Pecs Pécs Hungary 

出 版 物：《Open Journal of Immunology》 (免疫学期刊（英文）)

年 卷 期：2014年第4卷第4期

页      面：148-156页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：L-Selectin Pregnancy BALB/c CD62L NK Cells Lymphocyte Homing 

摘      要：Background: L-selectin (CD62L) is a cell surface adhesion molecule recently shown to play a critical role in determining endometrial receptivity and implantation in humans. By contrast, the L-selectin ligand is missing from the rodent endometrium. Interestingly, CD62L (L-selectin)-deficient BALB/c mice delivered significantly higher numbers of viable offspring than wild type controls via mechanisms yet to be defined. Methods: Nulliparous CD62L-deficient (8-10-week-old, n = 25) or wild type (n = 18) females were mated with 43 age-matched males. Animals were sacrificed at gestational day (GD) 9.5. Tissue samples were analyzed by immunostaining and flow cytometry. Results: Mating wild type and CD62L-deficient BALB/c mice revealed that the increased birth rate was due to the CD62L deficiency in females. Flow cytometric analysis demonstrated significant differences in the number of natural killer (NK) cells present in the uterus of pregnant CD62L- deficient mice compared to controls. Immunohistochemistry confirmed NK cell accumulation at the fetal-maternal interface. Discussion: Uterine NK cells have been shown to peak at GD 8-10 at the fetal-maternal interface. NK cells might regulate mouse fertility rates by facilitating development of the maternal spiral arteries, thereby stimulating the formation of larger vessels that facilitate intrauterine survival, however, their role is not obligate to spiral artery development. Conclusions: Diminished CD62L expression modified immune cell trafficking into the uterus of pregnant mice generating a microenvironment primarily dominated by NK cells resulting in improved embryonic survival rates.