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A murine model of dengue virus infection in suckling c57BL/6 and balb/c mice

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Animal Models and Experimental Medicine 2021年第1期4卷 16-26页

作者： Alana B.Byrne Ayelén G.García Jorge M.Brahamian Aldana Mauri Adrián Ferretti Fernando P.Polack Laura B.Talarico Fundación INFANT Buenos AiresArgentina consejo Nacional de Investigaciones científicas y Técnicas(cONIcET) Buenos AiresArgentina

Dengue is a significant public health concern across tropical and subtropical regions worldwide,principally causing disease in *** young children are at increased risk of severe manifestations of dengue *** mechanism of dengue disease in this population is not fully *** this study,we present a murine model of dengue virus primary infection in suckling c57BL/6 and balb/c mice in order to investigate disease ***-day-old c57BL/6 mice intraperitoneally infected with DENV-2 NGc were more susceptible to infection than balb/c mice,showing increased liver enzymes,extended viremia,dissemination to organs and histological alterations in liver and small ***,the immune response in DENV-infected c57BL/6 mice exhibited a marked Th1 bias compared to balb/c *** findings highlight the possibility of establishing an immunocompetent mouse model of DENV-2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study agerelated mechanisms of dengue pathogenesis.

关键词： balb/c c57BL/6 dengue virus mouse model suckling mice

Mouse-adapted SARS-coV-2 replicates efficiently in the upper and lower respiratory tract of balb/c and c57BL/6J mice

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Protein & cell 2020年第10期11卷 776-782页

作者： Jinliang Wang Lei Shuai chong Wang Renqiang Liu Ziruo Sun Dan Shan Jinying Ge Xijun Wang Ronghong Hua Gongxun Zhong Zhiyuan Wen Zhigao Bu Xijun He Xianfeng Zhang Zhigao Bu State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150069 China National High containment Laboratory for Animal Diseases control and Prevention Harbin 150069 China

Dear Editor,As of June,2020,more than ten million cases of cOVID-19 have been reported *** causative pathogen of the disease is a novel coronavirus named severe acute respiratory syndrome coronavirus 2(SARS-coV-2)(World Health Organization,2020).Animal infection models are important to characterize the infection,pathogenesis,and immunology of SARS・coV・2,as well as for the development of medications and vaccines against *** are particularly attractive animal models for their identical genetic background,reliable reproducibility,well characterized biology,and the huge availability of research reagents and knockout *** in inbreed mice such as balb/c and c57BL/6J(c57),which are widely used in research,are highly desired.

关键词： c57BL/6J balb/c respiratory

clinical hematological and biochemical parameters in Swiss,balb/c,c57BL/6 and B6D2F1 Mus musculus

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Animal Models and Experimental Medicine 2020年第4期3卷 304-315页

作者： Giorgio Silva-Santana Juliet cunha Bax Débora cristina Silva Fernandes Daniela Tendler Leibel Bacellar cleber Hooper Alexandre Alves Souza Oliveira Dias cristina Barbosa Silva Aline Moreira de Souza Simone Ramos Ricardo Alexandre Santos Thainara Ramos Pinto Mariana Antunes Ramão Ana Luíza Mattos-Guaraldi Health Sciences center Institute of Microbiology Paulo de GóesFederal University of Rio de JaneiroRio de JaneiroRJBrazil Laboratory of Diphtheria and corynebacteria of clinical Relevance Faculty of Medical SciencesUniversity of the State of Rio de JaneiroRio de JaneiroRJBrazil The collaborating centre for Reference and Research on Diphtheria/National Health Foundation/Ministry of Health Rio de JaneiroRJBrazil Laboratory of clinical Research and Molecular Diagnostic Prof.Marcílio Dias do Nascimento Department of Clinical Practice and PathologyFluminense Federal UniversityNiteróiRJBrazil Laboratory Bacterial Vaccines Department of ImmunologyNational Institute for Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroRJBrazil Institute of Science and Technology in Biomodels Oswaldo Cruz FoundationRio de JaneiroRJBrazil Institute of Biology Federal Fluminense UniversityNiteróiRJBrazil Biomedical center College of Medical SciencesUniversity of the State of Rio de JaneiroRio de JaneiroRJBrazil

Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental *** lineages of mice have been used to investigate diseases and new therapeutic strategies,and,consequently,hematological and biochemical tests in these laboratory animals are essential to validate scientific *** study seeks to establish reference values for hematological and biochemical parameters of four lineages of ***:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss(heterogeneous),balb/c and c57BL/6(isogenic),and B6D2F1(hybrid),totaling 160 *** were standardized using the systems pocH-100iV Diff™for 19 hematological parameters and VITROS®350 for 12 biochemical ***:Results are shown as means and standard deviation,grouped by lineage and *** the values obtained in this study with the values from previous studies,some variations were detected,which could be explained by differences in methodologies or individual ***:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary,and emphasises the importance of considering variations influenced by gender,lineage and genotype in the choice of the best experimental model.

关键词： B6D2F1 balb/c biochemical c57BL/6 hematological Swiss

Synergetic effect of Egyptian propolis in immunization of balb/c mice against bovine cysticercosis

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Asian Pacific Journal of Tropical Biomedicine 2015年第4期5卷 324-330页

作者： Omnia Mohamed Kandil Somia Ayesh Nassar Soad Mohamed Nasr Hatem Abdel Mawgoud Shalaby Seham Hendawy Faragalla Mohamed El Moghazy Department of Parasitology and Animal Diseases National Research Centre Biology Department College of Science and Humanity Studies Salman Bin Abdul Aziz University

Objective: To evaluate the synergetic effect of an ethanolic extract of Egyptian propolis in immunization of balb/c mice with Taenia saginata(T. saginata) crude antigen against bovine cysticercosis, with reference to its effects on liver and kidney ***: Sixty female mice balb/c strain weighing 20 to 25 g and 6-8 weeks old were randomly allocated into six groups of ten mice each. Mice in groups 1 and 2(G1 and G2) were immunized intraperitoneally with 100 μg of T. saginata crude antigen in 100 μL phosphate buffer saline emulsified in Freund's adjuvant. Besides, the mice in G2 were administered with propolis extract simultaneously with immunization. control mice were either administered with propolis extract(G3) or injected with the same volume of phosphate buffer saline emulsified in Freund's adjuvant(G4). The mice in G5 were non-immunized infected control while, those in G6 were non-immunized non-infected control. Two weeks after the last immunization, each mouse was challenged intraperitoneally with 5 000 oncospheres except those of G6. Ethanolic extract of propolis was prepared at a dose 50 mg/kg body ***: After 24 weeks of challenge, the mice in G2 showed the highest level of protection(100%), with no cyst being detected rather than mice in G1(33.3% protection). Additionally,the ELISA results, in this study, showed higher antibody titer in G2 with reduction the alteration in liver and kidney functions compared to ***: Egyptian propolis could increase the level of protection against experimental challenge infection with T. saginata eggs when administered simultaneously with immunization. Furthermore, it could enhance the production of antibodies to immunized antigen and decrease the alteration in liver and kidney functions.

关键词： Taenia saginata Vaccine balb/c mice ELISA Serum biochemistry

balb/c 3T3细胞体外转化实验及其在环境致癌物检测上的应用

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卫生研究 2009年第2期38卷 226-232页

作者：张磊曾毅中国疾病预防控制中心病毒病预防控制所北京100052

balb/c 3T3细胞体外转化实验是利用balb/c 3T3细胞在体外培养的环境下,模拟致癌物在动物体内致瘤过程进行致癌物筛选的一种技术,它的检测终点是正常细胞转化为恶性细胞时获得的恶性表型。本文就其方法的发展和改进,以及其在肿瘤启动剂... 详细信息

balb/c 3T3细胞体外转化实验是利用balb/c 3T3细胞在体外培养的环境下,模拟致癌物在动物体内致瘤过程进行致癌物筛选的一种技术,它的检测终点是正常细胞转化为恶性细胞时获得的恶性表型。本文就其方法的发展和改进,以及其在肿瘤启动剂和促癌剂检测上的应用进行了综述。

关键词： balb/c 3T3细胞细胞转化致癌物环境毒理

balb/c小鼠腹腔接种HBV表面S抗原疫苗引起的cTL反应(英文)

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第一军医大学学报 2003年第12期23卷 1242-1244页

作者：施理刘树人易学瑞孔祥平张宜俊 458医院全军肝病中心广东广州510602

目的研究balb/c小鼠免疫接种HBV表面S抗原疫苗(HBV small surface vaccine,S-HBsAg)能否引起特异性细胞毒T淋巴细胞(cTL)反应.方法分别给balb/c小鼠腹腔内接种0～6μg S-HBsAg,两周后加强一次,4周后分离小鼠脾细胞,体外用S-HBsAg特异性... 详细信息

目的研究balb/c小鼠免疫接种HBV表面S抗原疫苗(HBV small surface vaccine,S-HBsAg)能否引起特异性细胞毒T淋巴细胞(cTL)反应.方法分别给balb/c小鼠腹腔内接种0～6μg S-HBsAg,两周后加强一次,4周后分离小鼠脾细胞,体外用S-HBsAg特异性cTL表位多肽刺激;用特异性多肽、51cr标记的P815细胞作为靶细胞;4 h51cr释放实验检测cTL反应.结果分别接种0、0.65、1.25、2.5、5μg S-HBsAg的小鼠,其脾淋巴细胞cTL特异性释放率分别为31.21%±9.23%、42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%;只接受一次免疫接种的小鼠,其脾淋巴细胞cTL特异性释放率分别为27.34%±14.25%、32.27%±15.35%、56.28%±24.35%、44.34%±18.65%、40.76%±56%.结论balb/c(H-2d)小鼠腹腔内接种S-HBsAg引起剂量依赖性特异性cTL反应,加强免疫能够提高cTL反应.

关键词： balb/c 小鼠腹腔预防接种 HBV 表面S抗原疫苗 cTL反应异性细胞毒T淋巴细胞

c3d3-hcGβ融合蛋白免疫balb/c小鼠增强抗hcG-β T_H2型体液免疫效应

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中华微生物学和免疫学杂志 2004年第8期24卷 666-669页

作者：王秀丽李大金袁敏敏复旦大学附属妇产科研究所上海200011

目的　通过分子佐剂c3d3增强hcGβ避孕疫苗的免疫原性并实现免疫效应向TH2型体液免疫偏倚。方法　在cHO细胞中表达、纯化hcGβ、c3d3和hcGβ c3d3融合蛋白。分别用hcGβ c3d3融合蛋白、单用hcGβ、hcGβ联合c3d3和hcGβ加用弗氏完全佐... 详细信息

目的　通过分子佐剂c3d3增强hcGβ避孕疫苗的免疫原性并实现免疫效应向TH2型体液免疫偏倚。方法　在cHO细胞中表达、纯化hcGβ、c3d3和hcGβ c3d3融合蛋白。分别用hcGβ c3d3融合蛋白、单用hcGβ、hcGβ联合c3d3和hcGβ加用弗氏完全佐剂免疫balb c小鼠 ,共免疫 2次 ,间隔 4周。ELISA测定血清中抗hcGβ抗体滴度。末次免疫后 3周处死小鼠制备单个脾细胞悬液 ,体外用hcG刺激培养 4 8h ,ELISA检测上清中TH1型 (IFN γ、IL 2 )和TH2型 (IL 4、IL 10 )细胞因子分泌水平。结果　c3d3使hcGβ的免疫原性增强了 1995倍 ,c3d3的佐剂能力是弗氏完全佐剂的 10倍 (初次免疫 )～ 32倍 (再次免疫 )。借助c3d3分子佐剂 ,hcGβ c3d3融合蛋白可产生很明显的TH2型体液免疫优势效应。结论　分子佐剂c3d3可以大幅增强hcGβ避孕疫苗的免疫原性 ,使免疫效应向TH2型体液免疫偏倚。

关键词： c3d3-hcGβ融合蛋白免疫学 balb/c 小鼠抗hcG-βTH2型体液免疫效应免疫原性

balb/c乳鼠实验性感染汉坦病毒与脑神经细胞凋亡

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中华传染病杂志 2000年第2期18卷 95-97,I003页

作者：贺宏印刘俊彬杨守京刘彦仿火翠香解放军第一医院神经内科兰州730030 第四军医大学西京医院临床免疫科第四军医大学病理教研室

目的　应用原位末端标记技术对balb/c乳鼠脑组织的凋亡细胞进行检测 ,并对脑组织中汉坦病毒 (HV)感染和细胞凋亡的关系进行初步探讨。方法　HV陈株经腹腔接种balb/c乳鼠 ,感染后分别在 1、2、4、8、10和 14d取脑组织 ,4%多聚甲醛固定 ,... 详细信息

目的　应用原位末端标记技术对balb/c乳鼠脑组织的凋亡细胞进行检测 ,并对脑组织中汉坦病毒 (HV)感染和细胞凋亡的关系进行初步探讨。方法　HV陈株经腹腔接种balb/c乳鼠 ,感染后分别在 1、2、4、8、10和 14d取脑组织 ,4%多聚甲醛固定 ,常规制备 5 μm石蜡切片 ,应用原位杂交对乳鼠石蜡切片的病毒定位 ,用原位末端标记显示乳鼠脑组织中存在着凋亡细胞。结果　原位杂交检测结果显示 ,病毒主要累及大脑皮层、海马等部位 ,阳性信号主要分布于胞浆、紧靠核膜 ,原位末端标记的阳性细胞存在于神经细胞核内 ,在感染乳鼠脑组织中许多细胞被原位末端标记 ,呈胞核深染 ,弥漫分布于皮层、海马、白质等部位。结论　乳鼠脑组织中存在着凋亡细胞。

关键词： balb/c 汉坦病毒细胞凋亡

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balb／c小鼠脏器中锌锰铜含量的测定

解剖学报 1995年第4期26卷 422-424页

作者：曹东铉朴哲马延祥金艺兰崔玉顺玄希春延边医学院病理学教研室

对４０只２～３个月ＢＡＬＢ／ｃ小鼠的主要脏器用ＷＦＸ－Ⅱ型双光束原子吸收分光光度计测定了脏器中锌、锰、铜含量。结果表明，在主要脏器中锌、锰、铜含量不等。锌含量最多的是肝，最少的是脑和心；锰含量最多的是肝，最少的是子宫... 详细信息

对４０只２～３个月ＢＡＬＢ／ｃ小鼠的主要脏器用ＷＦＸ－Ⅱ型双光束原子吸收分光光度计测定了脏器中锌、锰、铜含量。结果表明，在主要脏器中锌、锰、铜含量不等。锌含量最多的是肝，最少的是脑和心；锰含量最多的是肝，最少的是子宫；铜含量最多的是子宫，最少的是脾。在雌雄小鼠之间无显著性差异。

关键词：微量元素内脏原子吸收分光光度计 balb/c