Synergistic effect between cryptotanshinone and antibiotics in oral pathogenic bacteria
Synergistic effect between cryptotanshinone and antibiotics in oral pathogenic bacteria作者机构：Department of Natural Products Research Institute of Jinan Red Ginseng Jinan South Korea Department of Nursing Dong-eui University Busan South Korea Department of Oral Microbiology and Institute of Oral Bioscience Chonbuk National University Jeonju South Korea
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2013年第4卷第2期
页 面：283-294页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Cryptotanshinone Antibacterial Activity Oral Bacteria Checker Board Method Time-Kill Method Synergistic Effect
摘 要：Cryptotanshinone (CT), a major tanshinone of medicinal plant Salvia miltiorrhiza Bunge, demonstrated effective in vitro antibacterial activity against all oral bacteria tested in this experiment. The antibacterial activities of CT against oral bacteria were assessed using the checkerboard and time-kill methods to evaluate the synergistic effects of treatment with ampicillin or gentamicin. The CT was determined against oral pathogenic bacteria with MIC and MBC values ranging from 0.5 to 16 and 1 to 64 μg/mL;for am- picillin from 0.0313 to 16 and 0.125 to 32 μg/mL;for gentamicin from 2 to 256 and 4 to 512 μg/mL respectively. The range of MIC50 and MIC90 were 0.0625 - 8 μg/mL and 1 - 64 μg/mL, respectively. The combination effects of CT with antibiotics were synergistic (FIC index 0.5) against tested oral bacteria except additive, Streptococcus sobrinus, S. criceti, and Actinobacillus actinomycetemcomitans (FIC index 0.75 - 1.0). The MBCs were shown reducing ≥4 - 8-fold, indicating a synergistic effect as defined by a FBCI of ≤0.5. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 3 - 6 h of treatment with the 1/2 MIC of CT, regardless of whether it was administered alone or with ampicillin or gentamicin. The results suggest that CT could be employed as a natural antibacterial agent against cariogenic and periodontopathogenic bac- teria.
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