BKI1 Regulates Plant Architecture through Coordinated Inhibition of the Brassinosteroid and ERECTA Signaling Pathways in Arabidopsis

作     者：Dongxu Wang Cangjing Yang Haijiao Wang Zhihua Wu Jianjun Jiang Jingjing Liu Zhuona He Fang Chang Hong Ma Xuelu Wang 

作者机构：State Key Laboratory of Genetic Engineering Department of Genetics School of Life Sciences Fudan University Shanghai 200438 China College of Life Science and Technology Huazhong Agricultural University No 1 Shizishan Street Wuhan Hubei 430070 China Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200438 China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2017年第10卷第2期

页      面：297-308页

核心收录：

学科分类：0710[理学-生物学] 090403[农学-农药学(可授农学、理学学位）] 07[理学] 071009[理学-细胞生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：Huazhong Agricultural University (to X.W.) the National Natural Science Foundation of China (to X.W.) 国家973计划 

主　　题：BKI1 receptor-like kinase ERECTA kinase inhibitor pedicel orientation crosstalk 

摘      要：Hundreds of leucine-rich repeat receptor-like kinases （LRR-RLKs） play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inactive status are essential but rarely studied. BKI1 is the only reported inhibitor of receptor kinases in Arabidopsis, which negatively regulates BRI1 in the brassinosteroid pathway. In this study, we found that BKI1 can also interact with another important LRR-RLK, ERECTA （ER）. Phenotypic analysis showed that BKI1 and ER together regulate plant architecture, including pedicel orientation, which is a newly reported phenotype in the BR- and ER-mediated developmental processes. Gene expression analysis revealed that BKI1 regulates a subset of ER-responsive genes. Kinase assays demonstrated that BKI1 inhibits ER kinase activity. In addition, the release of BKI1 inhibition on ER signaling relies largely on BRI1 activation. Our data provide significant insights into the regulation and activation of RLKs and suggest that BKI1 functions as a common suppressor of the BRI1 and ER signaling pathways.