The Apoptotic Effect of the Methanol Extract of <i>Polygonum cuspidatum</i>through Up-Regulation Death Receptor 5 and CHOP in HSC-2 Human Oral Cancer Cells

作     者：Hyun-Ju Yu Ji-Ae Shin Eun-Sun Choi Jae-Gyu Jeon Nam-Pyo Cho Sung-Dae Cho 

作者机构：Department of Oral Pathology School of Dentistry Institute of Oral Bioscience Chonbuk National University Jeonju South Korea 

出 版 物：《Journal of Cancer Therapy》 (癌症治疗（英文）)

年 卷 期：2012年第3卷第1期

页      面：1-6页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Polygonum cuspidatum Endoplasmic Reticulum Stress C/EBP Homologous Protein/Growth Arrest and the DNA Damage-Inducible Gene 153 (CHOP) Death Receptor 5 (DR5) Apoptosis Human Oral Cancer Cell 

摘      要：Polygonum cuspidatum is used as a traditional medicinal herb for the therapy of various diseases including several types of cancers. In the present study, we focused on addressing the anti-cancer activity and molecular mechanism of methanol extract of Polygonum cuspidatum (MEPC) in HSC-2 human oral cancer cells. The effect of MEPC on oral cancer cells was estimated by 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay, 4’-6-diamidino-2-phenylindole (DAPI) staining and Western blot analysis. MEPC inhibited the cell viability and induced apoptosis through the induction of death receptor (DR) 5. MEPC also increased the expression of C/EBP homologous protein/growth arrest and the DNA damage-inducible gene 153 (CHOP), a transcription factor induced by ER stress. Thus, we concluded that the induction of CHOP leading to DR5 up-regulation is required for the anti-cancer activity of MEPC in HSC-2 cells and MEPC may be a promising drug candidate for oral cancer.