Draft Genome Sequence of Mentha Iongifolia and Development of Resources for Mint Cultivar Improvement

作     者：Kelly J. Vining Sean R. Johnson Amirhossein Ahkami Iris Lange Amber N. Parrish Susan C. Trapp Rodney B. Croteau Shannon C.K. Straub Iovanna Pandelova B. Markus Lange 

作者机构：Department of Horticulture Oregon State University Corvallis OR 97331 USA M. J. Murdock Metabolomics Laboratory Institute of Biological Chemistry Washington State University Pullman WA 99164-6340 USA Department of Biology Hobart and William Smith Colleges Geneva NY 14456 USA Present address: Pacific Northwest National Laboratory Environmental Molecular Sciences Laboratory (EMSL) Richiand WA 99354 USA Present address: Medifluidics Inc. Fort Collins. CO 80525 USA 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2017年第10卷第2期

页      面：323-339页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 0710[理学-生物学] 0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 090501[农学-动物遗传育种与繁殖] 0902[农学-园艺学] 10[医学] 

基　　金：supported by grants from the Mint Industry Research Council supported by a grant from the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, U. S. Department of Energy 

主　　题：aromatic plant essential oil genome mint Verticillium wilt 

摘      要：The genus Mentha encompasses mint species cultivated for their essential oils, which are formulated into a vast array of consumer products. Desirable oil characteristics and resistance to the fungal disease Verti- cillium wilt are top priorities for the mint industry. However, cultivated mints have complex polyploid ge- homes and are sterile. Breeding efforts, therefore, require the development of genomic resources for fertile mint species. Here, we present draft de novo genome and plastome assemblies for a wilt-resistant South African accession of Mentha Iongifolia （L.） Huds., a diploid species ancestral to cultivated peppermint and spearmint. The 353 Mb genome contains 35 597 predicted protein-coding genes, including 292 disease resistance gene homologs, and nine genes determining essential oil characteristics. A genetic linkage map ordered 1397 genome scaffolds on 12 pseudochromosomes. More than two million simple sequence repeats were identified, which will facilitate molecular marker development. The M. Iongifolia genome is a valuable resource for both metabolic engineering and molecular breeding. This is exemplified by employing the genome sequence to clone and functionally characterize the promoters in a peppermint cultivar, and demonstrating the utility of a glandular trichome-specific promoter to increase expression of a biosynthetic gene, thereby modulating essential oil composition.