An Atypical Thioredoxin Imparts Early Resistance to Sugarcane Mosaic Virus in Maize

作     者：Qingqing Liu Huanhuan Liu Yangqing Gong Yongfu Tao Lu Jiang Weiliang Zuo Qin Yang Jianrong Ye Jinsheng Lai Jianyu Wu Thomas Lubberstedt Mingliang Xu 

作者机构：National Maize Improvement Centre of China China Agricultural University Beijing 100193 China Department of Agronomy Iowa State University Ames IA 50011 USA College of Agronomy Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop ScienceHenan Agricultural University Zhengzhou 450002 China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2017年第10卷第3期

页      面：483-497页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Key Research and Development Program of China USAID project iAGRI International Science and Technology Cooperation Program of China 

主　　题：maize sugarcane mosaic virus quantitative resistance gene h-type thioredoxin 

摘      要：Sugarcane mosaic virus （SCMV） causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. A major quantitative trait locus, Scmvl, has been identified to impart strong resistance to SCMV at the early infection stage. Here, we demonstrate that ZmTrxh, encoding an atypical h-type thioredoxin, is the causal gene at Scmvl, and that its transcript abundance correlated strongly with maize resistance to SCMV. ZmTrxh alleles, whether they are resistant or susceptible, share the identical coding/proximal promoter regions, but vary in the upstream regulatory regions. ZmTrxh lacks two canon- ical cysteines in the thioredoxin active-site motif and exists uniquely in the maize genome. Because of this, ZmTrxh is unable to reduce disulfide bridges but possesses a strong molecular chaperone-like activity. ZmTrxh is dispersed in maize cytoplasm to suppress SCMV viral RNA accumulation. Moreover, ZmTrxh- mediated maize resistance to SCMV showed no obvious correlation with the salicylic acid- and jasmonic acid-related defense signaling pathways. Taken together, our results indicate that ZmTrxh exhibits a distinct defense profile in maize resistance to SCMV, differing from previously characterized dominant or recessive potyvirus resistance genes.