Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii

作     者：Huan Zhao Qi Tang Changming Mo Longhua Bai Dongping Tu Xiaojun Ma 

作者机构：The Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences & Peking Union Medical College Hunan Provincial Key laboratory of Crop Germplasm Innovation and Utilization and National Chinese Medicinal Herbs(Hunan) Technology CenterHunan Agricultural University Guangxi Botanical Garden of Medicinal Plant 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2017年第7卷第2期

页      面：215-222页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Nature Science Foundation of China(No.81373914) National Key Technology Support Program(No.2011BAI01B03) Guangxi Natural Science Fundation(Nos.2013GXNSFDA019021 and 2012GXNSFAA053043) 

主　　题：Siraitia grosvenorii Triterpenoids Steroids Cloning Expression Squalene synthase Cycloartenol synthase Subcellular localization 

摘      要：Mogrosides and steroid saponins are tetracyclic triterpenoids found in Siraitia *** synthase(SQS) and cycloartenol synthase(CAS) are key enzymes in triterpenoid and steroid *** this study,full-length cDNAs of SgSQS and SgCAS were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction(RACE-PCR) *** SgSQS cDNA has a 1254 bp open reading frame(ORF) encoding 417 amino acids,and the SgCAS cDNA contains a 2298 bp ORF encoding 765 amino *** analysis showed that the deduced SgSQS protein has two transmembrane regions in the *** SgSQS and SgCAS have significantly higher levels in fruits than in other tissues,suggesting that steroids and mogrosides are competitors for the same precursors in *** in silico prediction and subcellular localization,experiments in tobacco indicated that SgSQS was probably in the cytoplasm or on the cytoskeleton,and SgCAS was likely located in the nucleus or *** results will provide a foundation for further study of SgSQS and SgCAS gene functions in ***,and may facilitate improvements in mogroside content in fruit by regulating gene expression.