Functional magnetic nanoparticles for facile viable but nonculturable bacteria separation and purification

作     者：Chunming Wang Huirong Lin Chengsong Ye 

作者机构：Key Lab of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2016年第10卷第6期

页      面：123-128页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：Acknowledgements This work was supported by the National Natural Science Foundation of China （Grant No. 21407141）. 

主　　题：Drinking water biosafety VBNC Nanoparticles Magnetic separation and purification 

摘      要：Viable But Nonculturable （VBNC） Bacteria, which represent a unique population of microorganisms in drinking water systems, have become a potential threat to human health. Current studies on VBNC ceils usually fail to obtain pure VBNC state bacteria, which may lead to inaccurate results. We therefore introduce a novel method of VBNC cell separation and puriticatlon in this paper. PAH-coated magnetic nanoparticles （MNPs） were synthesized and found to be capable of capturing and releasing bacterial cells with high efficiency. With the aid of an additional incubation step, VBNC cells were easily isolated and purified from normal bacteria using functional MNPs. Our method represents a new technique that can be utilized in studies of VBNCs.