Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells

作     者：Bin-Bin Li Guo-Liang Huang Hua-Hui Li Xia Kong Zhi-Wei He Li Bin-Bin;Huang Guo-Liang;Li Hua-Hui;Kong Xia;He Zhi-Wei

作者机构：Department of Pathophysiology Guangdong Medical University Dongguan Guangdong 523808 China Sino-American Cancer Research Institute Key Laboratory for Medical Diagnostics of Guangdong Province Guangdong Medical University Dongguan Guangdong 523808 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2017年第130卷第1期

页      面：93-99页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 090203[农学-茶学] 0902[农学-园艺学] 

基　　金：The present study was supported by grants from the National Natural Science Foundation of China (No. 81502411)  Science and Technology Planning Project of Guangdong Province (No. 2014A020212560)  and PhD Start-up Fund of Guangdong Medical University (No. B2014001) 

主　　题：Antitumor Agents Epigallocatechin-3-gallate MicroRNAs Nasopharyngeal Carcinoma 

摘      要：Background： Epigallocatechin-3-gallate （EGCG） has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma （NPC）, but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs （miRNAs） in NPC cells. Methods： Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis. Results： A total of 14 miRNAs exhibited 〉2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs. Conclusion： EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.