Gene editing nuclease and its application in tilapia

作     者：Minghui Li Deshou Wang 

作者机构：Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education China) Key Laboratory of Aquatic Science of Chongqing School of Life SciencesSouthwest University Chongqing 400715 China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2017年第62卷第3期

页      面：165-173页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the National Natural Science Foundation of China (31630082 and 31602314) the Natural Science Foundation Project of Chongqing,Chongqing Science and Technology Commission (cstc2015jcyj B0248 and cstc2013kjrc-tdjs80003) the Fundamental Research Funds for the Central Universities (XDJK2016C157) the China Postdoctoral Science Foundation (2015M570765,2016T90830) Chongqing Postdoctoral Science Foundation (Xm2015028) 

主　　题：Gene editing nuclease Genome engineering TilapiaSex determination 

摘      要：Gene editing nucleases including zinc-finger nucleases(ZFNs), transcription activator like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated(Cas) system(CRISPR/Cas9) provide powerful tools that improve our ability to understand the physiological processes and their underlying mechanisms. To date, these approaches have already been widely used to generate knockout and knockin models in a large number of species. Fishes comprise nearly half of extant vertebrate species and provide excellent models for studying many aspects of biology. In this review, we present an overview of recent advances in the use of gene editing nucleases for studies of fish species. We focus particularly on the use of TALENs and CRISPR/Cas9 genome editing for studying sex determination in tilapia.