Acetylcholinesterase Biosensor Based on Poly(diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

作     者：Xia Sun Zhili Gong Yaoyao Cao Xiangyou Wang 

作者机构：College of Agriculture and Food EngineeringShandong University of Technology 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2013年第5卷第1期

页      面：47-56页

核心收录：

学科分类：0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 0817[工学-化学工程与技术] 070205[理学-凝聚态物理] 08[工学] 080202[工学-机械电子工程] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(No.30972055,31101286) Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China(No.2011GB2C60020) Shandong Provincial Natural Science Foundation,China(No.Q2008D03) 

主　　题：Biosensor Acetylcholinesterase Multi-walled carbon nanotubes Graphene Poly(diallyldimethy lammonium chloride) 

摘      要：In this paper, an amperometric acetylcholinesterase(ACh E) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly(diallyldimethy-lammonium chloride)-multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode(GCE) surface, then ACh E was immobilized onto the modified GCE to fabricate the ACh E biosensor. The morphologies and electrochemistry properties of the prepared ACh E biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the ACh E activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.