Ankfy1 is dispensable for neural stem/precursor cell development

作     者：Chao Weng Man Ding Lian-sheng Chang Ming-xin Ren Hong-feng Zhang Zu-neng Lu Hui Fu 

作者机构：Department of NeurologyRenmin Hospital of Wuhan University Department of Anatomy and EmbryologySchool of Basic Medical SciencesWuhan University Department of PathologyCentral Hospital of Wuhan Hubei-MOST KLOS & KLOBME 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第11期

页      面：1804-1809页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：Dr.Hui Fu was supported by the National Natural Science Foundation of China,No.81371338 by Open Research Fund Program of Hubei-MOST KLOS & KLOBME Dr.Zu-neng Lu was supported by grants from Health and Family Planning Commission of Hubei Province scientific research project,No.WJ2015MA007 

主　　题：nerve regeneration Ankfyl neural development genetic background protein function gene knockout neural stem/precursor cells embryo neural regeneration 

摘      要：There are few studies on the membrane protein Ankfyl. We have found Ankfyl is specifically expressed in neural stem/precursor cells during early development in mice （murine）. To further explore Ankfyl function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfyl null mice during development and in adults up to 2 months old. However, Ankfyl gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfyl protein is dispensable in neural stem/precursor ceils, but could be critical for early embryonic murine development, depending on the genetic background.