ISOLATION AND STRUCTURE OF RANAMARGARIN,A NEW TACHYKININ FROM THE SKIN OF CHINESE FROG Rana margaratae

作     者：唐易全 田盛海 吴时祥 华家柽 吴贯夫 赵尔宓 陆一安 朱应麒 邹冈 TANG YI-QUAN TIAN SHENG-HAI WU SHI-XIANG HUA JIA-CHENG WU GUAN-FU ZHAO ER-MI LU YX-AN ZHU YING-QI AND ZOU GANG (TSOU KANG (Shanghai Institute of Materia Medica, Academia Sinica)

作者机构：Shanghai Institute of Materia Medica Academia Sinica Chengdu Institute of BiologyAcademia Sinica Shanghai Centre of BiotechnologyAcademia Sinica 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1989年第32卷第5期

页      面：570-579页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

主　　题：ranamargarin Rana margaratae amphibian skin HPLC sequence analysis. 

摘      要：A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C_(18) and HPLC from methanol extracts of the skin of the Chinese frogRana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH_2. This structure has been confirmed by synthesis. The peptide is the largest among the amphibian tachykinins and its N-terminal amino acids are quite different from those of the other tachykinins. The formation of the sulfoxide and peak-splitting of ranamargarin during purification procedures are briefly discussed.