Comparison of ESI–and APCI–LC–MS/MS methods:A case study of levonorgestrel in human plasma

作     者：Rulin Wang Lin Zhang Zunjian Zhang Yuan Tian 

作者机构：Key Laboratory of Drug Quality Control and PharmacovigilanceMinistry of EducationChina Pharmaceutical University State Key Laboratory of Natural MedicineChina Pharmaceutical University 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2016年第6卷第6期

页      面：356-362页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 0702[理学-物理学] 10[医学] 

主　　题：Levonorgestrel LC–MS/MS Pharmacokinetics Electrospray ionization Atmospheric pressure chemical ionization 

摘      要：Electrospray ionization(ESI) and atmospheric pressure chemical ionization(APCI) techniques for liquid chromatography–tandem mass spectrometry(LC–MS/MS) determination of levonorgestrel were *** consideration of difference in ionization mechanism,the two ionization sources were compared in terms of LC conditions,MS parameters and performance of *** sensitivity for detection of levonorgestrel with ESI was 0.25 ng/m L which was lower than 1 ng/m L with *** effects were evaluated for levonorgestrel and canrenone(internal standard,IS) in human plasma,and the results showed that APCI source appeared to be slightly less liable to matrix effect than ESI *** an overall consideration,ESI was chosen as a better ionization technique for rapid and sensitive quantification of *** optimized LC–ESI–MS/MS method was validated for a linear range of 0.25–50 ng/m L with a correlation coefficient ≥*** intra-and inter-batch precision and accuracy were within 11.72% and 6.58%,*** application of this method was demonstrated by a bioequivalence study following a single oral administration of 1.5 mg levonorgestrel tablets in 21 Chinese healthy female volunteers.