Preparation, stability and pharmacokinetics evaluation of lipid microspheres loading a promising antitumor candidate, Timataxel

作     者：Yan Li Haibing He Qiao Wang Xing Tang 

作者机构：Shenyang Pharmaceutical University 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2016年第11卷第6期

页      面：771-779页

核心收录：

学科分类：100702[医学-药剂学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Program for Liaoning Innovative Talents in University 

主　　题：Timataxel Lipid microspheres Stability assessment Pharmacokinetics 

摘      要：Timataxel(13-(N-Boc-3-i-butylisoserinoyl-4,10-β-diacetoxy-2-α-benzoyloxy-5-β-20-epoxy-1,13-α-dihydroxy-9-oxo-19-norcyclopropa[g]tax-11-ene)), used to be called TM-2, is a novel semi-synthetic promising candidate for cancer treatment. However the preformulation study showed that TM-2 was insoluble and chemically instable in water, which would limit its application. This study aimed at the preparation of Timataxel lipid microspheres(TM-2 LMs)and investigated the difference between TM-2 LMs and TM-2 solution in pharmacokinetics. In this work, the final formulation was as follows: 0.10%(w/v) TM-2;10.00%(w/v) oil phase(long chain triglyceride:media chain triglyceride = 2.50%:7.50%);1.40%(w/v) phospholipid;0.02%(w/v) NaH2 PO4;2.25%(w/v) glycerin and water to a total volume of 100 *** particle size distribution, content and entrapment efficacy were 205.0 ± 43.3 nm, 101.00% and 99.12%, respectively. TM-2 LMs were stable during storage at 25 °C for 3 months, even under the condition of 60 °C and 4500 lx for 10 d. Phosphatidylethanolamine(PE) in phospholipid may contribute to the stability of TM-2 LMs. The pharmacokinetic parameters for TM-2 LMs were as follows: AUC(0-∞) 3663.71 μg/l h and the clearance 2.26 l/h/kg. As for solution, these parameters were 1712.52 μg/l h and 4.77 l/h/kg, respectively. The t1/2 of TM-2 LMs was similar to TM-2 solution. The pharmacokinetic results indicated that the AUC of TM-2 LMs was larger, the clearance was smaller than that of TM-2 solution. In a word, lipid microspheres were a promising drug delivery system for TM-2.