TAA family contributes to auxin production during de novo regeneration of adventitious roots from Arabidopsis leaf explants

作     者：Beibei Sun Lyuqin Chen Jingchun Liu Xuening Zhang Zhongnan Yang Wu Liu Lin Xu 

作者机构：National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032China College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 China University of Chinese Academy of Sciences 19A Yuquan RoadBeijing 100049 China Department of Plant and Microbial Biology Ztirich-Basel Plant Science Center University of Zurich 8008 Zurich Switzerland 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2016年第61卷第22期

页      面：1728-1731页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by the National Basic Research Program of China (973 Program,2014CB943500/2012CB910503) the National Natural Science Foundation of China (91419302/31422005) Youth Innovation Promotion Association CAS 

主　　题：De novo root organogenesis TAA1TAR2 Plant regeneration Auxin biogenesis Arabidopsis 

摘      要：Many differentiated plant organs have the ability to regenerate into a new plant after detachment via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, wounding first induces endogenous auxin production in mesophyll cells. Auxin is then polar transported to, and accumulates in, regenerationcompetent cells near the wound to trigger the cell-fate transition. The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS(TAA) family proteins and the YUCCA(YUC) family proteins catalyze two successive biochemical steps in auxin biogenesis, and YUCs have been shown to be involved in auxin production in mesophyll cells during de novo root organogenesis. In thisstudy, we show that the TAA family is also required for adventitious rooting. Inhibition of TAA blocked adventitious root formation from leaf explants. Intriguingly,whereas YUC1 and YUC4 have been shown to be highly induced by wounding, TAA genes retained consistent expression levels before and after leaf ***, we suggest that TAAs and YUCs are both required for auxin biogenesis in leaf explants, but they play different roles in regeneration. While YUC1 and YUC4 function in response to wounding to catalyze the ratelimiting step in auxin biosynthesis, TAAs probably serve as abiding and basal enzymes during de novo root organogenesis from leaf explants.