Investigations of the interaction between cuprous oxide nanoparticles and Staphylococcus aureus

作     者：SHEN ChengLing LI YuanFang QI WenJing HUANG ChengZhi 

作者机构：Key Laboratory on Luminescence and Real-Time Analysis of Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China College of Life ScienceSouthwest UniversityChongqing 400715China College of Pharmaceutical ScienceSouthwest UniversityChongqing 400715China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2009年第52卷第7期

页      面：1028-1032页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 30570465 & 20425517) 

主　　题：cuprous oxide nanoparticles Staphylococcus aureus minimal inhibitory concentration (MIC) flow cytometry (FCM) 

摘      要：In this study, cuprous oxide nanoparticles of 30–50 nm in size were prepared in the presence of cetyltrimethylammonium bromide (CTAB). By taking Staphylococcus aureus (S.a), which always causes a variety of suppurative infections and toxinoses in humans, as a model bioparticle, the negative bioeffect of nano-Cu2O on S.a cells was evaluated, and minimal inhibitory concentration (MIC) was determined by imitating the MIC of antibiotics. Cellularity and bactericidal effect were measured by flow cytometry (FCM), dark field light scattering imaging and SEM photography. The results showed that nano-Cu2O particles may, by absorbing on the cell surface, impair the cell wall, damage the cell membrane, and finally increase permeability of the cell membrane, thus leading to a decrease in the viability of bacteria in the nano-Cu2O solution.