Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection

作     者：HuaZHANG WenRuiJIN 

作者机构：StateKeyLaboratoryofMicrobialTechnologyShandongUniversityJinan250100 SchoolofChemistry-andChemicalEngineeringShandongUniversityJinan250100 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2003年第14卷第9期

页      面：952-954页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China the State Key Laboratory of Microbial Technology Shandong University 

主　　题：Capillary electrophoresis laser-induced fluorescence detection single cell analysis amino acid. 

摘      要：A novel approach for analysis of amino acids in individual erythrocytes was established. In this method, the derivatization reagent was introduced into the living cells by electroporation. After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection.