REGULATION OF NITRATE REDUCTASE ACTIVITY IN RICE

作     者：林振武 汤玉玮 LIN ZHENWU TANG YUWEI (Shanghai Institute of Plant Physiology, Academia Sinica)

作者机构：Shanghai Institute of Plant Physiology Academia Sinica 

出 版 物：《Science in China,Ser.B》 (中国科学B辑(英文版))

年 卷 期：1988年第31卷第10期

页      面：1195-1203页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China 

主　　题：nitrate reductase nitrate reductase-mRNA rice regulation property. 

摘      要：The properties of NR from rice varieties Yuanfengzao and Jingyin 127 are the same in molecular weight of native enzyme and subunit, optimum pH, Km value for substrates NO3- and NADH, stability in vitro and immunological characteristics. The decay rate in vitro of the activity of the partially purified NR from Yuanfengzao is 20％ lower than that from Jingyin 127. The results from antiserum titration and rocket immunoelectrophoresis indicated that the amount of NR in Yuanfengzao is about 1.5 times more than that in Jingyin 127. Further study on in vivo incorporation of 3H-labeled amino acids into NR revealed that the rate of NR synthesis in Yuanfengzao is obviously higher than that in Jingyin 127. In vitro translation of mRNA showed that the products of NR-mRNA per unit of total mRNA of Yuanfengzao is much more than that of Jingyin 127. It is concluded that, therefore, NR activity in rice is regulated through NR synthesis and NR-inhibitory protein（s） system, the former is further controlled at the lev