Expression of Catalytic Domain of Protein Tyrosine Phosphatase 1B and Preparation of Its Polyclonal Antibody

作     者：SHI Dong-lei DONG Hong-bo ZHU Zhi-cheng SUN Mei SU Ji-quan ZHANG Xing-yi XU Yue-chi FU Xue-qi 

作者机构：Department of Thoracic Surgery The Second Hospital of Jilin University Jilin University Changchun 130012 P. R. China College of PharmacyJilin University Changchun 130012 P. R. China Department of Pathology The Second Hospital of Jilin UniversityJilin University Changchun 130012 P. R. China College of Life Sciences Jilin University Changchun 130012 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2007年第23卷第2期

页      面：204-207页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 100102[医学-免疫学] 0703[理学-化学] 10[医学] 

基　　金：the Science and Technology Department of Jilin Province(No 20060563) 

主　　题：Protein tyrosine phosphatase IB Catalytic domain Polyclonal antibody 

摘      要：This study focuses on the expression of human protein tyrosine phosphatase 1B （PTP1 B） catalytic domain （△PTP1B） and preparation of polyclonal antibody against △PTP1B. △PTP1B gene was PCR amplified with the cDNA of human PTP1B as the template, and cloned into the pT7 expression vector. The recombinant pT7-△PTP1B was expressed in E. eoli Rosetta（ DE3 ） host cells and purified. The antiserum was prepared by immunizing rabhit with purified recombinant △PTP1B. The polyclonal antibody against △PTP1B was purified by PVDF immobilized antigen affinity chromatography. △PTP1B was correctly cloned, expressed, and purified as confirmed by PCR, DNA sequence analysis, SDS-PAGE western blotting, and ILPLC. The titer and sensitivity of the antibody were 1：2500（ volume ratio） and 0. 1 ng, respectively. This study provides an important basis for further studying the biological function of PTP1B and its relationship with human diseases.