TOTAL SYNTHESIS OF YEAST ALANINE TRANSFER RIBONUCLEIC ACID

作     者：王德宝 郑可沁 裘慕绥 梁镇和 吴仁龙 陈常庆 汪恩壁 朱莹书 申庆祥 余允华 汪猷 陈海宝 杨再完 陆蕴华 陈慎 王贵海 胡美浩 WANG DEBAO ZHENG KEQIN QIU MUSUI, LIANG ZHENHE WU RENLONG CHEN CHANGQING (Shanghai Institute of Biochemistry, Academia Sinica) WANG ENBI ZHU YINGSHU SHEN QINGXIANG YU YUNHUA (Shanghai Institute of Cell Biology, Academia Sinica) WANG YOU, CHEN HAIBAO YANG ZAIWAN LU YUNHUA (Shanghai Institute of Organic Chemistry, Academia Sinica) CHEN SHEN WANG GUIHAI (Institute of Biophysics, Academia Sinica, Beijing) HU MEIHAO (Department of Biology, Peking University) AND SHANGHAI NO. 2 REAGENT FACTORY

作者机构：Shanghai Institute of Biochemistry Academia Sinica Shanghai Institute of Cell Biology Shanghai Institute of Organic Chemistry Institute of Biophysics Beijing Department of Biology Peking University 

出 版 物：《Science in China,Ser.B》 (中国科学B辑(英文版))

年 卷 期：1983年第26卷第5期

页      面：464-481页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 0708[理学-地球物理学] 100214[医学-肿瘤学] 0704[理学-天文学] 10[医学] 

主　　题：In tRNA TOTAL SYNTHESIS OF YEAST ALANINE TRANSFER RIBONUCLEIC ACID Chen OC 

摘      要：By a combination of chemical and enzymatic methods, small oligonucleotides with lengths varying from 2 to 8 nucleotides were synthesized from mononucleotides. The small oligonucleotides were then ligated with T4 RNA ligase into six laxge ligonucleotides （9 to 19 nucleotides long） which were further ligated to form two half molecules with 35 and 41 nucleotides respectively, Finally, the two synthetic half molecules were annealed and ligated to obtain the whole molecule of yeast alanine tRNA (tRNAyAla). Prior to this, two semi-syntheses were performed, i. e. ligation of the synthetic 5’-half molecule with the natural 3’-half molecule and that of the natural 5’-half molecule with the synthetic 3’-half molecule.