Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness
Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness作者机构：Peking-Yale Joint Center for Plant Molecular Genetics and Agro-biotechnology State Key Laboratory of Protein and Plant Gene Research Peking -Tsinghua Center for Life Sciences College of Life Sciences Peking University Beijing 100871 China Department of Biology Wilkes University Wilkes-Barre PA 18766 USA Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520-8104 USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2015年第8卷第4期
页 面：622-630页
核心收录：
学科分类：0710[理学-生物学] 08[工学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 0902[农学-园艺学] 081201[工学-计算机系统结构] 0812[工学-计算机科学与技术（可授工学、理学学位）]
基 金：This work was supported by grants to H.C. from the National Natural Science Foundation of China （31271294）, the National Program on Key Basic Research Project of China （973 Program： 2011CB100101）, the National High Technology Research and Development Program of China （863 Pro- gram： 2012AA10A304）, the Ministry of Agriculture of China （948 Program： 2011-G2B）, and State Key Laboratory of Protein and Plant Gene Research and grants to X.W.D. from the National Natural Science Foundation of China （31330048, U1031001）, the National Program on Key Basic Research Project of China （973 Program： 2012CB910900）, Peking-Tsinghua Center for Life Sciences, and State Key Laboratory of Protein and Plant Gene Research. ACKNOWLEDGMENTS We thank Renbo Yu, Jie Dong, and other laboratory members for their constructive discussion and help. No conflict of interest declared.
主 题：DET1 DELLA gibbereltic acid （GA） photomorphogenesis Arabidopsis
摘 要：Arabidopsis De-etiolated 1 （DET1） is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid （GA） also participates in this process, and plants deficient in GA synthesis or signaling show a partially de.etiolated phenotype in darkness. However, how DET1 and the GA pathway work in concert in repressing photomorphogenesis remains largely unknown. In this study, we found that the abundance of DELLA proteins in detl-1 was increased in comparison with that in the wildtype plants. Mutation in DET1 changed the sensitivity of hypocotyl elongation of mutant seedlings to GA and paclobutrazol （PAC）, an inhibitor of GA synthesis. However, we did not find obvious differences between detl-1 and wild-type plants with regard to the bioactive GA content or the GA signaling upstream of DELLAs. Genetic data showed that removal of several DELLA proteins suppressed the detl-1 mutant phenotype more obviously than GA treatment, indicating that DET1 can regulate DELLA proteins via some other mechanisms. In addition, a large-scale transcriptomic analysis revealed that DET1 and DELLAs play antagonistic roles in regulating expression of photosynthetic and cell elongation-related genes in etiolated seedlings. Taken together, our results show that DET1 represses photomorphogenesis in darkness in part by reducing the abundance of DELLA proteins.
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