Effects of IGF-1 and oxLDL on expression of phosphatase PHLPP1 in vascular smooth muscular cells
Effects of IGF-1 and oxLDL on expression of phosphatase PHLPP1 in vascular smooth muscular cells作者机构：Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China
出 版 物：《Journal of Geriatric Cardiology》 (老年心脏病学杂志（英文版）)
年 卷 期：2009年第6卷第4期
页 面：237-240页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071003[理学-生理学]
主 题：PH domain leucine-rich repeat protein phosphatasel (PHLPP1) insulin-like growth factor-l oxidized low density lipoprotein(oxLDL) vascular smooth muscle cells
摘 要：Objective To investigate the effects of insulin-like growth factor-1 （IGF-1） and oxidized low density lipoprotein （oxLDL） on expression ofphosphatase PHLPP 1 in vascular smooth muscle cells （VSMCs）. Methods Rabbit aortic VSMCs were cultured. VSMCs proliferation ability was determined by measuring cell number and mitochondrial dehydrogenase （MD） activity with MTT assay. Western blot was used to detect the protein expression ofphosphatase PHLPP1. Results IGF-1 （100ug/L） increased cell number and MD activity to 3.02 and 3.59 times of that in control group, oxLDL（501xg/ml） elevated the above two parameters to 2.03 and 2.91 times respectively. Western blot showed that IGF-1 and oxLDL inhibited the expression of PHLPPI to 39.27% and 40.26% of the control group （P〈0.01 ）. Conclusion IGF- 1 and oxLDL may enhance the proliferation of VSMCs by decreasing the expression ofphosphatase PHLPP 1.
维普期刊数据库