Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA

作     者：Yah Jing SU Xiong Hui WEI 

作者机构：Department of Applied Chemistry College of Chemistry and Molecular Engineering Peking University Beijing 100871 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2006年第17卷第5期

页      面：691-694页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：Chelerythrine CT-DNA intercalation thermodynamic. 

摘      要：The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×10^5 and a binding site size of 3,4 base pairs at 25 ℃. The intercalative interaction is exothermic with a Van＇t Hoff enthalpy of -33.7 kJ/mol. In addition, △G =-32.9 kJ/mol, and △S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.