Characterization of catechol 1,2-dioxygenase from cell extracts of Sphingomonas xenophaga QYY

作     者：M.Salah Uddin 

作者机构：Department of Genetics and Breeding University of RajshahiRajshahi-6205 Bangladesh 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2009年第52卷第5期

页      面：615-620页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 50608011) the 39th Postdoctoral Funds of China (Grant No. 20060390983) 

主　　题：catechol 1 2-dioxygenase cell extracts characterization Sphingomonas xenophaga 

摘      要：Sphingomonas xenophaga QYY, capable of growing significantly on more than ten kinds of aromatic compounds as sole carbon source, was used to study characterization of catechol 1,2-dioxygenase (C12O) in cell extracts. Characterization of the crude C12O showed that the maximum activity was obtained at 40–70°C and pH 7.8–8.8. Metal ions had different influences on the activity of crude C12O. It was suggested that strain QYY possessed an inducible and ferric-dependent C12O. Kinetic studies showed that the value of V max and K m was 0.25 μmol catechol/L/mg protein/min and 52.85 μmol/L, respectively. In addition, the partial purification of C12O was achieved by a HiTrap Q Sepharose column chromatography.