Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells
Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells作者机构:Affiliated Hospital of Hebei University of Engineering
出 版 物:《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志(英文版))
年 卷 期:2016年第9卷第5期
页 面:480-484页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:supported by Handan Science and Technology Bureau(Project No.1323108088)
主 题:Mi R-34a Laryngeal squamous carcinoma Hep2 cells Proliferation Apoptosis Invasion
摘 要:Objective: To discuss the effect and mechanism of miR-34 a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells. Methods: The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34 a mimics and miR-34 a NC. The MTT, colony-forming assay, Hoechst staining and Annexin V-PI double staining flow cytometry were employed to detect the effect of miR-34 a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34 a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RTPCR assay to defect the effect of miR-34 a mimics on the expression of survivin and Ki-67 m RNA in laryngeal squamous carcinoma Hep2 cells. Results: Compared with miR-34 a NC group, the cell viability in miR-34 mimics group was significantly decreased(P0.01), the cell apoptosis rate was significantly increased(P0.01), the abilities of cell migration and invasion were significantly reduced(P0.01) and the expression of survivin and Ki-67 m RNA was significantly decreased(P0.01). Conclusions: The increased expression of miR-34 a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down-regulated expression of survivin and Ki-67.