Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells

作     者：Ju-Xiang Wang Qing-Jun Zhang Shi-Geng Pei Bao-Liang Yang 

作者机构：Affiliated Hospital of Hebei University of Engineering 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2016年第9卷第5期

页      面：480-484页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Handan Science and Technology Bureau(Project No.1323108088) 

主　　题：Mi R-34a Laryngeal squamous carcinoma Hep2 cells Proliferation Apoptosis Invasion 

摘      要：Objective: To discuss the effect and mechanism of miR-34 a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells. Methods: The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34 a mimics and miR-34 a NC. The MTT, colony-forming assay, Hoechst staining and Annexin V-PI double staining flow cytometry were employed to detect the effect of miR-34 a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34 a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RTPCR assay to defect the effect of miR-34 a mimics on the expression of survivin and Ki-67 m RNA in laryngeal squamous carcinoma Hep2 cells. Results: Compared with miR-34 a NC group, the cell viability in miR-34 mimics group was significantly decreased(P0.01), the cell apoptosis rate was significantly increased(P0.01), the abilities of cell migration and invasion were significantly reduced(P0.01) and the expression of survivin and Ki-67 m RNA was significantly decreased(P0.01). Conclusions: The increased expression of miR-34 a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down-regulated expression of survivin and Ki-67.