Human cytomegalovirus-encoded US28 may act as a tumor promoter in colorectal cancer
Human cytomegalovirus-encoded US28 may act as a tumor promoter in colorectal cancer作者机构：Department of Gastroenterology The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University Department of Surgical Oncology Wenzhou Central Hospital Department of General Surgery The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University Department of Pathology Wenzhou Medical University
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2016年第22卷第9期
页 面：2789-2798页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by The Zhejiang Provincial Natural Science Foundation of China,No.LY15H160059 Zhejiang Provincial Medical and Health Science and Technology Project,No.2016KYB192 the Wenzhou Municipal Science and Technology Bureau,No.Y20140691
主 题：Human cytomegalovirus US28 Colorectal cancer Prognosis Proliferation Invasion
摘 要：AIM: To assess human cytomegalovirus-encoded US28 gene function in colorectal cancer(CRC) pathogenesis.METHODS: Immunohistochemical analysis was performed to determine US28 expression in 103 CRC patient samples and 98 corresponding adjacent noncancerous samples. Patient data were compared by age, sex, tumor location, histological grade, Dukes stage, and overall mean survival time. In addition, the US28 gene was transiently transfected into the CRC LOVO cell line, and cell proliferation was assessed using a cell counting kit-8 assay. Cell cycle analysis by flow cytometry and a cell invasion transwell assay were also carried out.RESULTS: US28 levels were clearly higher in CRC tissues(38.8%) than in adjacent noncancerous samples(7.1%)(P = 0.000). Interestingly, elevated US28 amounts in CRC tissues were significantly associated with histological grade, metastasis, Dukes stage, and overall survival(all P 0.05); meanwhile, US28 expression was not significantly correlated with age, sex or tumor location. In addition, multivariate Coxregression data revealed US28 level as an independent CRC prognostic marker(P = 0.000). LOVO cells successfully transfected with the US28 gene exhibited higher viability, greater chemotherapy resistance, accelerated cell cycle progression, and increased invasion ability.CONCLUSION: US28 expression is predictive of poor prognosis and may promote CRC.
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