Genotoxicity of ferric oxide nanoparticles in Raphanus sativus：Deciphering the role of signaling factors,oxidative stress and cell death

作     者：Quaiser Saquib Mohammad Faisal Abdulrahman A.Alatar Abdulaziz A.Al-Khedhairy Mukhtar Ahmed Sabiha M.Ansari Hend A.Alwathnani Mohammad K.Okla Sourabh Dwivedi Javed Musarrat Shelly Praveen Shams T.Khan Rizwan Wahab Maqsood A.Siddiqui Javed Ahmad 

作者机构：Zoology Department College of Sciences King Saud University P.O. Box 245S Riyadh 114S1 Saucli Arabia A.R. Al-Jeraisy Chair for DNA Research Zoology Department College of Sciences King Saud University P.O. Box 2455 Riyadh 11451Saudi Arabia Department of Botany & Microbiology College of Sciences King Saucl University P.O. Box 2455 Riyaclh 11451 Saudi Arabia Department of Agricultural Microbiology Faculty of Agricultural Sciences Aligarh Muslim University AIigarh 202002 India Baba Ohulam Shah Badshah Uniuersity Rajouri 185131Jammu and Kashmir India Division of Plant Pathology Indian Agricultural Research Institute New Delhi 110012 India 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2016年第28卷第9期

页      面：49-62页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：funded by the National Plan for Science,Technology and Innovation(MAARIFAH) King Abdul Aziz City for Science and Technology,Kingdom of Saudi Arabia,award number 12-BIO2919-02 

主　　题：Ferric oxide nanoparticles Nanotoxicity DNA damage Apoptosis Oxidative stress Phytotoxicity 

摘      要：We have studied the genotoxic and apoptotic potential of ferric oxide nanoparticles（Fe_2O_3-NPs） in Raphanus sativus（radish）.Fe_2O_3-NPs retarded the root length and seed germination in *** sections of treated roots showed subcellular localization of Fe_2O_3-NPs,along with the appearance of damaged mitochondria and excessive *** cytometric analysis of Fe_2O_3-NPs（1.0 mg/m L） treated groups exhibited 219.5%,161%,120.4% and 161.4% increase in intracellular reactive oxygen species（ROS）,mitochondrial membrane potential（ΔΨm）,nitric oxide（NO） and Ca2＋influx in radish protoplasts.A concentration dependent increase in the antioxidative enzymes glutathione（GSH）,catalase（CAT）,superoxide dismutase（SOD） and lipid peroxidation（LPO） has been *** assay showed a concentration dependent increase in deoxyribonucleic acid（DNA） strand breaks in Fe_2O_3-NPs treated *** cycle analysis revealed 88.4% of cells in sub-G1 apoptotic phase,suggesting cell death in Fe_2O_3-NPs（2.0 mg/m L） treated *** together,the genotoxicity induced by Fe_2O_3-NPs highlights the importance of environmental risk associated with improper disposal of nanoparticles（NPs） and radish can serve as a good indicator for measuring the phytotoxicity of NPs grown in NP-polluted environment.