Heat-induced mortality and expression of heat shock proteins in Colorado potato beetles treated with imidacloprid

作     者：Jie Chen Ai Kitazumi Jasper Alpuerto Andrei Alyokhin Benildo de los Reyes 无

作者机构：School of Biology and Ecology University of Maine Orono ME 04469 USA Department of Entomology LouisianaState University Baton Rouge LA 70803 USA. 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2016年第23卷第4期

页      面：548-554页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：funded in part by the USDA-CSREES-NRI Award 

主　　题：chemical stress climate change heat stress insecticide resistance integratedpest management Leptinotarsa decemlineata (Say) 

摘      要：The Colorado potato beetle is an important pest of solanaceous plants in the Northern Hemisphere. Better understanding of its physiological responses to temperature stress and their interactions with still-prevalent chemical control has important implications for the management of this insect. We measured mortality and expression of the Hsp70 heat shock proteins in the Colorado potato beetle larvae exposed to sublethal concentration of the commonly used insecticide imidacloprid, and to supraoptimal temperatures. Both turned out to be significant stress factors, although induction of Hsp70 by imidacloprid observed in the present study was low compared to its induction by the heat. The two factors also interacted with each other. At an extreme temperature of 43 ~C, exposure to a sublethal dose of imidacloprid resulted in a significant rise in larval mortality, which was not observed at an optimal temperature of 25 ~C. Heat-stressed larvae also failed to respond to imidacloprid by producing more Hsp70. These findings suggest that when field rates of insecticides become insufficient for killing the exposed beetles under optimal temperature conditions due to the evolution of resistance in beetle populations, they may still reduce the probability of resistant beetles surviving the heat shock created by using propane flamers as a rescue treatment.