Theoretical Proof and Empirical Confirmation of a Continuous Labeling Method Using Naturally ^13C-Depleted Carbon Dioxide
Theoretical Proof and Empirical Confirmation of a Continuous Labeling Method Using Naturally ^13C-Depleted Carbon Dioxide作者机构：Environmental Studies University of California Santa Cruz CA 95064 USA
出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)
年 卷 期：2007年第49卷第3期
页 面：401-407页
核心收录：
学科分类：09[农学] 0903[农学-农业资源与环境] 090302[农学-植物营养学]
基 金：Supported by the National Research Initiative of the USDA Cooperative State Research Education and Extension Service (2003-35107-13716) and Kearney Foundation of Soil Science. Publication of this paper is supported by the National Natural Science Foundation of China (30624808) and Science Publication Foundation of the Chinese Academy of Sciences.Acknowledgements The authors thank Prarthna Naidu and Justine Keylor for their assistance with watering plants and laboratory work and David Harris for isotope analysis
主 题：carbon flux CO2 isotope rhizosphere tracers
摘 要：Continuous Isotope labeling and tracing is often needed to study the transformation, movement, and allocation of carbon in plant-soil systems. However, existing labeling methods have numerous limitations. The present study Introduces a new continuous labeling method using naturally ^13C-depleted CO2. We theoretically proved that a stable level of ^13C-CO2 abundance in a labeling chamber can be maintained by controlling the rate of CO2-free air Injection and the rate of ambient airflow with coupling of automatic control of CO2 concentration using a CO2 analyzer. The theoretical results were tested and confirmed in a 54 day experiment in a plant growth chamber. This new continuous labeling method avoids the use of radioactive ^14C or expensive ^13C-enrlched CO2 required by existing methods and therefore eliminates issues of radiation safety or unaffordable isotope cost, as well as creating new opportunities for short- or long-term labeling experiments under a controlled environment.
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