Pharmacological advantages of melatonin in immunosenescence by improving activity of T lymphocytes

作     者：Yeong-Min Yoo Su Kil Jang Gwang-Hoon Kim Jung-Youl Park Seong-Soo Joo 

作者机构：Department of Biomedical EngineeringCollege of Health ScienceYonsei UniversityWonjuGangwon-do 220-710Republic of Korea College of Life ScienceGangneung-Wonju National UniversityGangneungGangwon-do 210-702Republic of Korea Industry-Academic Cooperation FoundationHanbat National UniversityDaejeon 305-719Republic of Korea 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2016年第30卷第4期

页      面：314-321页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：supported by High Value-added Food Technology Development Program,Ministry of Agriculture,Food and Rural Affairs(MAFRA grant number 113034-3) 

主　　题：melatonin aging CD4＋ naive CD4 melatonin receptor 

摘      要：Melatonin plays a critical role in regulating photoperiodic signals and has recently been shown to decrease immunosenescence with age. In this study, we examined whether melatonin activates T lymphocytes as major adaptive immune cells in in vitro and in vivo models. Splenocytes, CD4＋, and naive CD4 T lymphocytes were isolated from the spleen of BALB/c mice and the cell population patterns and mRNA profiles associated with T cell activation （CD28 and p21） and the melatonin receptor （MT1A and MT1B） were assessed. The T cell activation- related proteins Ki67 and Bcl2 were also evaluated to confirm the relationship between gene and protein levels. Our data clearly revealed that CD28, p21, MT 1 A, and MT 1B mRNA were highly expressed in the presence of melatonin. Co-culture of CD4＋ T lymphocyte and peritoneal macrophage 7 days after melatonin administration to young and aged mice significantly increased APRIL mRNA, suggesting induction or maintenance of T lymphocyte responses. We also found that the intracellular amount of Ki67 and Bcl2 proteins were significantly upregulated in aged CD4＋ T lymphocytes, suggesting enhancing T cell proliferation and ling-term maintenance of memory T cells. Taken together, we conclude that melatonin supplementation may enhance immunity in aged individuals by upregulating immunosenescence indices in association with T lymphocytes and may be an attractive pharmacological candidate for aged and immunocompromised individuals.