Extracellular matrix from human umbilical cordderived mesenchymal stem cells as a scaffold for peripheral nerve regeneration

作     者：Bo Xiao Feng Rao Zhi-yuan Guo Xun Sun Yi-guo Wang Shu-yun Liu Ai-yuan Wang Quan-yi Guo Hao-ye Meng Qing Zhao Jiang Peng Yu Wang Shi-bi Lu 

作者机构：Institute of OrthopedicsChinese PLA General HospitalBeijingChina Beijing Key Laboratory of Regenerative Medicine in OrthopedicsBeijingChina The Neural Regeneration Co-innovation Center of Jiangsu ProvinceNantongJiangsu ProvinceChina Department of OrthopedicsFirst Affiliated HospitalSchool of MedicineShihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina Department of OrthopedicsCangzhou Central HospitalCangzhouHebei ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第7期

页      面：1172-1179页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,Grant No.31170946 the National Program on Key Basic Research Project of China(973 Program) Grant No.2012CB518106 and No.2014CB542201 the Special Project of the“Twelfth Five-year Plan”for Medical Science Development of PLA,No.BWS13C029 

主　　题：umbilical Schwann regeneration scaffold neurotrophic biomaterial niche fibronectin glial engineered 

摘      要：The extracellular matrix,which includes collagens,laminin,or fibronectin,plays an important role in peripheral nerve ***,a Schwann cell-derived extracellular matrix with classical biomaterial was used to mimic the neural ***,extensive clinical use of Schwann cells remains limited because of the limited origin,loss of an autologous nerve,and extended in vitro culture *** the present study,human umbilical cord-derived mesenchymal stem cells（h UCMSCs）,which are easily accessible and more proliferative than Schwann cells,were used to prepare an extracellular *** identified the morphology and function of h UCMSCs and investigated their effect on peripheral nerve *** with a non-coated dish tissue culture,the h UCMSC-derived extracellular matrix enhanced Schwann cell proliferation,upregulated gene and protein expression levels of brain-derived neurotrophic factor,glial cell-derived neurotrophic factor,and vascular endothelial growth factor in Schwann cells,and enhanced neurite outgrowth from dorsal root ganglion *** findings suggest that the h UCMSC-derived extracellular matrix promotes peripheral nerve repair and can be used as a basis for the rational design of engineered neural niches.