High Expression of p300 in Human Breast Cancer Correlates with Tumor Recurrence and Predicts Adverse Prognosis

作     者：Xiang-sheng Xiao Mu-yan Cai Jie-wei Chen Xin-yuan Guan Hsiang-fu Kung Yi-xin Zeng Dan Xie 

作者机构：State Key Laboratory of Oncology in South ChinaGuangzhou 510060China Department of Breast OncologyGuangzhou 510060China Department of PathologySun Yat-sen University Cancer CenterGuangzhou 510060China The Chinese University of Hong KongHong KongChina 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2011年第23卷第3期

页      面：201-207页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 07[理学] 08[工学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 081202[工学-计算机软件与理论] 

基　　金：supported by a grant from the National Natural Science Foundation of China (No. 30901709) the National "973" Basic Research Program of China (No. 2010CB529400 and 2010CB912802) 

主　　题：Breast cancer p300 Tumor recurrence Prognosis 

摘      要：Objective：Transcriptional coactivator p300 has been shown to play a variety of roles in the transcription process and mutation of p300 has been found in certain types of human cancers.However,the expression dynamics of p300 in breast cancer （BC） and its effect on BC patients＇ prognosis are poorly understood.Methods：In the present study,the methods of tissue microarray and immunohistochemistry （IHC） were used to investigate the protein expression of p300 in BCs.Receiver operating characteristic （ROC） curve analysis,Spearman＇s rank correlation,Kaplan-Meier plots and Cox proportional hazards regression model were utilized to analyze the data.Results：Based on the ROC curve analysis,the cutoff value for p300 high expression was defined when the H score for p300 was more than 105.High expression of p300 could be observed in 105/193 （54.4%） of BCs,in 6/25 （24.0%） of non-malignant breast tissues,respectively （P=0.004）.Further correlation analysis showed that high expression of p300 was positively correlated with higher histological grade,advanced clinical stage and tumor recurrence （P0.05）.In univariate survival analysis,a significant association between high expression of p300 and shortened patients＇ survival and poor progression-free survival was found （P0.05）.Importantly,p300 expression was evaluated as an independent prognostic factor in multivariate analysis （P0.05）.Conclusion：Our findings provide a basis for the concept that high expression of p300 in BC may be important in the acquisition of a recurrence phenotype,suggesting that p300 high expression,as examined by IHC,is an independent biomarker for poor prognosis of patients with BC.