Determination of the Geographical Origin of Fruits by Using 26S rDNA Fingerprinting of Yeast Communities by PCR-DGGE： An Application to Shea Tree Fruits

作     者：Aly EI Sheikha 

作者机构：Department of Food Science and Technology Minufiya University Shibin El Kom 32511 Egypt UMR Qualisud French Agricultural Research Centre for International Development （CIRAD） 34398 Montpellier Cedex 5 France 

出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)

年 卷 期：2010年第4卷第6期

页      面：9-15页

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 080203[工学-机械设计及理论] 0802[工学-机械工程] 

主　　题：Biological bar-code PCR-DGGE Shea tree fruits yeast communities origin. 

摘      要：International trade intensifies the process of globalization which has extended to the entire planet and extends to the entire planet. In view of the difficulties of installing these documentary systems in developing countries, in particularly the countries of sub-Saharan Africa, the new strategies of traceability emerge. Among the new tools of tracing the products of vegetable origin, a ＂biological code bar＂ based on the analysis of the DNA of micro-organisms present on the fruits is an interesting tool. A molecular technique employing 26S rDNA profiles generated by PCR-DGGE was used to detect the variation in yeast community structures of Shea tree fruits （Vitellaria paradoxa） from Senegal, Ghana and Mali. When the 26S rDNA profiles were analyzed by image analysis and multivariate analysis, distinct microbial communities were detected on Shea tree fruits. The band profiles of yeasts from different countries were specific for each location and could be used as a bar code to discriminate the origin of the fruits. This method is a new traceability tool which provides fruit products with an unique biological bar code and makes it possible to trace back the fruits to their original location.