Down-regulation of DPH2L gene during cellular differentiation/apoptosis:Use of mRNA differential display

作     者：Nicoletta Ferrari Giovanni Levi 

作者机构：Laboratory of Molecular Biology National Institute for Cancer ResearchLaboratory of Molecular Biology National Institute for Cancer Research Genova Italy Genova Italy 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：1999年第44卷第6期

页      面：496-503页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：State "863" High-Tech Project 

主　　题：mRNA differential display retinoic acids cell differentiation apoptosis tumor suppressor gene. 

摘      要：To characterize the genes associated with differentiation/apoptosis induced by all-trans retinoic acid (ATRA) in human lung cancer cells, mRNA differential display was employed. Six cDNA fragments have been isolated, and one of them corresponds to a sequence-known gene DPH2L with unknown function, which was first isolated from the critical region of deletion on chromosome 17p13.3 in human ovarian carcinoma, and regarded as a candidate tumor suppressor gene. Results show that DPH2L is a wide expressed gene, and has another major transcript besides the previously reported 2.3 kb transcript. It is proved that the DPH2L gene is dawn-regulated during differentiation or apoptosis in several kinds of cancer cells induced by all-trans retinoic acid and N-(4-hydroxyphenyl) retinamide (4HPR). This may suggest that DPH2L does not play a role as a tumor suppressor gene, on the contrary, its down-regulation may be functionally involved in the transition from cell growth to differentiation or apoptosis.