The Preliminary Study of Glucocorticoid Receptor Gene in Chinese Patients with Glucocorticoid-induced Glaucoma

作     者：Yehong Zhuo, Jian Ge, Yan Guo, Mingkai LinZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou 510060 , China 

作者机构：Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：1999年第15卷第1期

页      面：46-50页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：by grants from Nature Science Fundation of China Guangdong province (No.39800163 and No.980112) 

主　　题：糖皮质激素受体 基因 青光眼 

摘      要：Background: To study the glucocorticoid receptor (GR) and the associated gene regulation in the pathogenesis of glucocorticoid- induced glaucoma (GIG) in Chinese ***: The trabecular cells of normal individuals and patients with GIG were cultured in vitro. By using polymerase chain reaction (PCR),gene fragments on GR DNA binding sites of trabecular cells were amplified. The product was detected by gel ***: The trabecular cells were cultured successfully in normal individuals and patients with GIG in vitro. A single PCR product was obtained in both two groups with the same size of 545 base ***: There is not any difference in gene on the GR DNA binding sites between normal individuals and patients with GIG. The results suggest the difference in mRNA or other functional genes. Eye Science 1999 ; 15 ; 46 - 50.